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Fig. 2. Expression patterns of GFP-tagged LIM-SR (A,D), ${alpha}$ -actinin in the same fields (B,E), and the merged images with LIM-SR and ${alpha}$ -actinin in the green and red channels, respectively (C, F). In some cells LIM-SR is diffusely distributed (A), but it is most often concentrated at the cell periphery (arrows) and along fibers continuously stained for ${alpha}$ -actinin (arrowheads). The transfected cells in A and D exhibit LIM-SR expression levels that do not differ by more than a factor of two when normalized to cell area. LIM-SR expression appears to have disrupted ${alpha}$ -actinin organization into periodic striations when compared with neighboring untransfected cells.

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