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Fig. 4. Expression patterns of GFP-tagged IB-SR (A,D), {alpha}-actinin in the same fields (B,E), and the merged images with IB-SR and {alpha}-actinin in the green and red channels, respectively (C,F). In many areas IB-SR was co-localized with {alpha}-actinin at Z-lines (arrowheads), but in some areas IB-SR appeared to be excluded from mature Z-lines (D-F, asterisk). IB-SR fluorescence often extended beyond the Z-lines, marking the entire I-bands (A-C, inset). In addition, IB-SR fluorescence appeared continuous in immature regions where myofibrils appeared to be fusing laterally (arrows).





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