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Fig. 7. The apoptosis-mediating effect of Nma111p depends on its serine-protease activity. Indirect immunofluorescence localisation of ProtA-Nma111p (A) and ProtA-Nma111p-S235C (B) in yeast cells grown to mid-log phase. Sytox-Green nucleic-acid stain of ProtA-Nma111p (C), ProtA-Nma111p-S235C cells (D) and ${Delta}$ nma111 cells (E), as well as visualisation of DNA-strand breaks by TUNEL staining in ProtA-Nma111p (F), ProtA-Nma111p-S235C (G) and ${Delta}$ nma111 cells (H). Confocal fluorescence micrographs and coincident fluorescence/differential-interference contrast-images are shown. Scale bars, 5 µm (A-E), 10 µm (F-H). (I) Survival of ProtA-Nma111p, ProtA-Nma111p-S235C and ${Delta}$ nma111 cells grown to mid-log phase after incubation with H₂O₂ for 4 hours at indicated concentrations.

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