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Fig. 3. Subcellular localisation of PKC{alpha} (A) and PKC{epsilon} (B) in unstimulated and TPA or TRH stimulated pituitary glands of transgenic mice exhibiting GFP-GH expressing cells. Pituitary glands were removed from the animals, incubated in HamF10 culture medium containing or not 100 nM TPA or TRH for 1 hour, fixed in paraformaldehyde, cut into 40 µm sections and immunostained with antibodies raised against the {alpha} and {epsilon} PKC isoforms. Both enzymes are cytoplasmic under unstimulated conditions and selectively translocated to cell-cell contacts of green fluorescent GH cells as well as a subpopulation of GFP-GH-negative cells.





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