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Fig. 5. The GD(E)E motif located in the V3 region of both PKC ${alpha}$ and ${epsilon}$ is critical but not sufficient for the targeting to cell-cell contacts. GFP-tagged wild-type and mutated PKC ${alpha}$ , ${epsilon}$ and ${delta}$ were transiently transfected in GH3B6 cells. Cells were stimulated or not with 100 nM TPA. A selective translocation to cell-cell contacts was only observed for the wild-type PKC ${alpha}$ and ${epsilon}$ upon TPA stimulation. This targeting was abolished when the GD(E)E motif within the V3 region of both isoforms was mutated (mutants PKC ${alpha}$ -D294G and PKC ${epsilon}$ -E374G). The presence of this motif is however not sufficient to target PKC ${delta}$ to cell-cell contacts.

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