|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 1. Time course of skeletal muscle regeneration after barium chloride-induced injury. (A) Skeletal muscle regeneration is completed 10-15 days after the injection of 60 µl of 1.2% w/v BaCl2 into mice TA muscle. Degenerating myofibers together with abundant mononuclear cells, mostly inflammatory and muscle precursor cells, are observed the first days after the injection. Regenerated fibers, with nuclei in a central position, appear by the fourth or fifth day after the injection. Haematoxylin and Eosin stained cross sections from control and regenerating muscles at different times after barium chloride injection are presented in the first column, and the double-detection of 
-dystroglycan by immunofluorescence and AChRs by labeling with rhodamine-conjugated -bungarotoxin is presented in the second and third column, respectively. -Dystroglycan staining surrounds normal and regenerating muscle fibers and is associated with the remnants of basement membranes 3 days after the injection. Clusters of AChR can be detected throughout the whole process, associated with necrotic fibers or regenerating myotubes, but their number is reduced after barium chloride induced damage. Scale bars: 50 µm. (B) Transient protein expression of myogenin and embryonic myosin during skeletal muscle regeneration. Aliquots of extracts containing equivalent amounts of protein, obtained from control TA on different days after injury were subjected to SDS-PAGE, transferred to a nitrocellulose membrane and analyzed by western blot as described in the Materials and Methods.
|
