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Fig. 4. Early up-regulation of syndecan-3 mRNA during skeletal muscle regeneration. (A) Double-labeling with anti-embryonic myosin F1.652 antibody (EM; detected with fluorescein-conjugated secondary antibodies, green) and specific antibodies (detected with rhodamine-conjugated secondary antibodies, red) against laminin (Lam), syndecan-3 (Syn3) and perlecan (Per) was performed in TA sections from muscles injected with barium chloride 4 days before. The arrowhead indicates elongated cells beneath the basal lamina of intact myofibers that express embryonic myosin, probably nascent myotubes, being formed by fusion of activated satellite cells. Syndecan-3 is detected on the surface of these cells expressing embryonic myosin. Perlecan, on the contrary, has a pattern similar to laminin staining. Nuclei were stained with Hoechst 33258 (blue) to facilitate morphological identification. The bar indicates 50 µm. (B) A significant and early up-regulation of syndecan-3 mRNA during skeletal muscle regeneration was detected by northern blot, whereas only a small increase on the fifth day was observed for glypican-1 mRNA content.
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