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Fig. 5. Proliferation capacity in damaged muscle is not impaired in AN-10i as compared to C2C12i myoblasts 24 hours after transplantation. Representative microphotographs of skeletal muscle transverse sections 1 day after grafting with C2C12i (A,B) and AN-10i (C-D) myoblasts, showing X-gal staining of ß-galactosidase (A,C) and immunofluorescent detection of BrdU labeling in the same sections (B,D). Arrowheads indicate nuclei positive for both BrdU and ß-galactosidase. Scale bar: 50 µm. (E) No difference was found between C2C12i and AN-10i myoblasts when BrdU labeling indexes were quantified in ß-galactosidase-positive cells. (F) C2C12i and AN-10i proliferate at similar rates in normal growing conditions in cell culture.





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