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Fig. 5. Response of cell migration and traction forces to local application of Gd3+ near the leading edge. Phase contrast images (A-C) of a migrating NIH3T3 fibroblast, recorded immediately before (A) and at specified time points after the application of 100 µM Gd3+ near the leading edge (arrows, B,C), showed no inhibition of local protrusive activities (arrows), although the cell body failed to migrate forward. Asterisks mark a fiduciary reference point on the substratum. The corresponding traction stress images (D-F), with different colors representing the magnitude, from 4.25x101 dynes/cm2 (violet) to >4.50x104 dynes/cm2 (red), showed a global decrease in traction stress following the local Gd3+ application. Scale bar: 20 µm.





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