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Fig. 6. Response of cell migration and traction forces to local application of Gd3+ near the trailing edge. Phase contrast images (A-C) of a migrating NIH3T3 fibroblast, recorded immediately before (A) or at specified time points after the application of 100 µM Gd3+ near the trailing edge, showed no apparent effect on cell morphology or migration. Asterisks mark a fiduciary reference point on the substratum. The corresponding traction stress images (D-F), with different colors representing the magnitude, from 7.63x101 dynes/cm2 (violet) to >4.75x104 dynes/cm2 (red), showed no apparent effect on traction stress following the local Gd3+ application. Scale bar: 20 µm.





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