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Fig. 2. In vitro interaction of DLin-7 with members of different MAGUK subfamilies. (A) Proteins that were tested for interaction in a yeast two-hybrid assay. Subregions expressed as baits or preys are indicated as bars together with the positions of the first and last amino acid residues. The symbols used for the various protein domains are summarized in the box below. See Discussion for an interpretation of the cryptic L27 domain of Sdt. (B) Results of the yeast two-hybrid assay. The strength of each interaction was classified by the time period between the onset of the ß-Gal filter assay and the detection of a clearly visible color reaction; ++++, within 30 minutes or less; +++, within 1 hour; ++, within 3 hours; +, within 5 hours; –, more than 5 hours. The empty bait vector pGBKT7 served as a negative control. The weak interaction between DLin-7 and Dlg-S97 was also observed in the reverse bait-prey combination.





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