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Fig. 8. Isoform-specific interaction between Dlg-S97 and DLin-7. (A-F) Rescue of DLin-7 at dlg-mutant NMJs on muscle 12 upon postsynaptic expression of EGFP-Dlg-A versus EGFP-Dlg-S97. DLin-7-specific immunofluorescences of w1118 (A) and dlg^XI-2 (B) are shown for reference. (C) Gal4-C57-driven EGFP-Dlg-A was targeted to NMJs and to nuclei (arrow), whereas co-staining reveals no obvious enrichment for DLin-7 at either compartment (D). Postsynaptic targeting of EGFP-Dlg-S97 (E) was accompanied by effective restoration of DLin-7 (F). Each image represents a stack of 17 optical sections taken at 0.5 µm steps. (G) Co-immunoprecipitation assay performed on body wall muscle extracts from w1118- (lanes 1, 3) and UAS-Flag-DLin-7/Gal4-C57 (lanes 2, 4) 3rd instar larvae using anti-Flag antibody. Input control samples (lanes 1, 2) and immunoprecipitated protein samples (lanes 3, 4) were analyzed by western blotting using anti-DlgPDZ1+2. Note the enrichment of the 120 kDa isoform in lane 4.

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