spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1. Syntaxin 8 and CFTR expression in the CHO-CFTR cell line and CHO-2T clone. (A) A monoclonal antibody raised against syntaxin 8 was used to study syntaxin 8 expression levels and localization in CHO cells expressing either CFTR (CHO-CFTR) or both CFTR and syntaxin 8 (CHO-2T). Specific labeling was visualized using a TRITC-conjugated secondary antibody. Bars, 30 µm. (B) Equal amounts of protein (10 µg) were subjected to SDS-PAGE followed by immunoblotting. Syntaxin 8 overexpression was confirmed in the CHO-2T clone. (C) Cell lysates containing an equal amount of protein were subjected to immunoprecipitation with CFTR antibody. The presence of CFTR in CHO-CFTR and in CHO-2T cells was revealed by immunoblotting.





Right arrow Return to article