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Fig. 4. Analysis by patch-clamp assay of the CFTR chloride channel activity in the presence of syntaxin 8. Typical whole-cell currents recorded from a CHO-CFTR cell (A) and from a Syn8 and CFTR transfected CHO-2T cell (B) in the absence or presence of 5 µM forskolin in the bath. Cells capacitances are 30 pS and 27 pS in A and B, respectively. (C) Averaged current-voltage relationships from 12 CHO-CFTR cells and seven CHO-2T cells in the presence of 5 µM forskolin. (D) Histograms showing current densities measured at +60 mV for the different experimental conditions indicated at the bottom of each column. The number of experiments is indicated on the graph. ***P<0.0001.





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