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Fig. 8. CFTR and syntaxin 8 belong to the same protein complex. (A) Co-immunoprecipitation of CFTR with Syn8 in CHO-2T and HT29-CL19A cell lysates. Immunoprecipitated proteins were revealed after western blot with antibodies against CFTR. Bands B and C represent immature and mature forms of CFTR, respectively. CFTR antibody and nonimmune mouse IgG were used as positive and negative immunoprecipitation controls, respectively. (B) Co-immunoprecipitation of Syn8 with CFTR in CHO-2T and HT29-CL19A cell lysates. Immunoprecipitated proteins were revealed after western blot with antibodies against Syn8. CHO-2T and HT29 cells lysates were used as a positive control for western blot. Syn 8 antibody and nonimmune mouse IgG were used as positive and negative immunoprecipitation controls, respectively.





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