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Fig. 9. In vitro interactions between CFTR and endosomal SNARE proteins. (A) In vitro binding of GFP-CFTR with different recombinant cytosolic domains of syntaxin 8: GST-Syn8{Delta}TM, GST-Syn8[1-99] and GST-Syn8[99-209]. (B) In vitro binding of GFP-CFTR with different recombinant endosomal SNARE proteins: GST-Syn7{Delta}TM, GST-VAMP8{Delta}TM and GST-vti1b{Delta}TM. (A,B) Glutathione-sepharose-immobilized GST or GST-fusion proteins were incubated with COS-7+GFP-CFTR cell extracts. Bound proteins were studied by anti-GFP immunoblot. COS-7+GFP-CFTR lysate was used as a GFP-CFTR control; GST-Syn1A{Delta}TM and GST alone were used, respectively, as positive and negative pull-down controls.





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