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Fig. 4. Glucose induces the processing of SREBP-1c precursor (p) and the nuclear translocation of the mature form (m). Contracting myotubes were cultured in 5 mM glucose without serum for 48 hours. (A) Cytoplasmic (top) and nuclear (bottom) extracts were prepared at 0 minutes, 15 minutes, 30 minutes or 60 minutes after the addition of 25 mM glucose. The western blots represent three separate experiments. (B,C) Detection of the precursor and nuclear forms of SREBP-1c by immunofluorescence in contracting myotubes. In absence of 25 mM glucose, SREBP-1c precursor (green) was observed in the cytoplasm (a,b,g,h). Following the addition of 25 mM glucose, nuclear translocation of SREBP-1c was detected in myotubes after 30 minutes (c,d,i,j) and remained up to 60 minutes (e,f,k,l). Nuclei were counterstained with DAPI. Scale bars, 20 µm (B), 5 µm (C).





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