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Fig. 3. Phase contrast (A) and fluorescent (B) images of myofilament associated {epsilon}-PKC. {epsilon}-PKC is activated with 50 µM arachidonic acid (AA) and the activated {epsilon}-PKC is associated with myofilaments near Z-lines as indicated by arrows in the overlay image (C). A confocal image of a skinned myocyte decorated with {epsilon}-PKC, an anti-{epsilon}-PKC primary antibody and Alexa 488 secondary antibody (D) Magnification x1000.





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