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Fig. 4. Isoform specificity of PKC binding to cardiac myofibrils. (A) Western blots of myofibril pellets after incubation with ${alpha}$ -PKC, ${delta}$ -PKC or ${epsilon}$ -PKC. Below each lane is indicated the PKC activators used to stimulate binding. na, No activators. AA, 50 µM arachidonic acid. AA + DAG, 50 µM AA plus 25 µM dioctanoylglycerol. ${epsilon}$ M is ${epsilon}$ -PKC gel/blot marker, and ${delta}$ M is ${delta}$ -PKC gel/blot marker.

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