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Fig. 5. Inhibition of {epsilon}-PKC binding to cardiac myofibrils by synthetic peptides. (A) A Western blot shows {epsilon}-PKC in the myofibril pellet is reduced in a concentration-dependent manner by the octapeptide, EAVSLKPT. The F-actin binding hexapeptide LKKQET had no effect of {epsilon}-PKC binding to myofibrils over the same concentration range. {epsilon}-PKC concentration was 100 nM and peptide concentrations in µM are given above each lane. (B) Summary of peptide inhibition of {epsilon}-PKC binding to cardiac myofibrils. Bars represent mean±s.e.m. of five experiments.





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