spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 6. Properties of {epsilon}-PKC binding to isolated F-actin. (A) Summary of {epsilon}-PKC binding to F-actin in the absence and presence of 50 µM arachidonic acid (AA). The line with solid circles (in the presence of AA) represents a fit to: B/Bmax=[{epsilon}-PKC]/([{epsilon}-PKC] + EC50), with EC50=110 nM and Bmax=18 pmol (or 180 pmol/mg protein). The stoichiometry of binding was 18 pmols {epsilon}-PKC bound per 2 nmoles actin monomers or a 1/110 ratio. Open circles represent {epsilon}-PKC with F-actin in the absence of AA. (B) Summary of inhibition of {epsilon}-PKC binding to F-actin by synthetic peptides. Data is represented as mean±s.e.m. from five experiments.





Right arrow Return to article