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Fig. 5. (A) CD45.2-PE⁺ BM cells purified by FACS and hybridized with the Y-chromosome probe by FISH (red). A red-orange outline at the periphery of the nuclei indicates the CD45.2-PE signal that persisted after FISH. Nuclei are counter-stained with DAPI (blue). (B) CD45.2-PE-negative muscle cells sorted by FACS and hybridized with the Y-probe by FISH. No hybridization signals were observed. (C) FACS analysis of a muscle sample, which was taken 8 weeks after BMT from a mouse injected with e-GFP plus BM. SP (2.2%) and MP (71.4%) cell gates are shown. (D) Percentage of GFP⁺ BM-derived cells in muscle MP cells and in muscle SP cells (E). (F) More than 98% of muscle SP cells from an e-GFP transgenic mouse are positive for e-GFP.

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