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Fig. 4. Lack of colocalization of the GTP-bound Rab5a(Q79L) mutant with either wt or palmitoylation-deficient GAD65 in the soma of primary neurons. Confocal images of the soma of hippocampal neurons co-transfected with plasmids encoding either wt (A) or palmitoylation-deficient GAD65-GFP (B) together with HA-tagged constitutively active mutant of Rab5a(Q79L). Neurons were triple immunolabeled for GFP, HA, and the Golgi marker GM130. (A,B) Neither Wt GAD65-GFP nor palmitoylation-deficient GAD65-GFP are detected in the giant endosomes in the soma containing Rab5a(Q79L). Both Wt GAD65-GFP and palmitoylation-deficient GAD65-GFP are localized in the Golgi compartment, immunostained for GM130 but devoid of Rab5a(Q79L). Scale bar, 10 µm.





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