spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 7. Accumulation of wt but not palmitoylation-deficient GAD65 in neurite tips in the presence of the Rab5(S34N) mutant. Confocal images of hippocampal neurons 24 hours after transfection with plasmids encoding HA-tagged Rab5a(S34N) (A-D) and wt GAD65-GFP (A,B) or palmitoylation-deficient GAD65-GFP (C,D) and double immunolabeled for GFP and HA. Enlarged frames show (from left to right) immunolabeling for GFP, immunolabeling for HA, and overlay, respectively. (A) Wt GAD65 accumulates with Rab5a(S34N) in the tip of dendrites. (B) View of the distal axon of the neuron shown in A reveals accumulation of wt GAD65 together with Rab5a(S34N) at the tips of the branched axon. (C) Palmitoylation-deficient GAD65 does no accumulate with Rab5a(S34N) at the tip of dendrites. (D) View of the distal axon of the neuron shown in C reveals the absence of palmitoylation-deficient GAD65 in accumulates of Rab5a(S34N) at the tip of axons. Scale bars, 10 µm.





Right arrow Return to article