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Fig. 9. Depletion of dLBR and lamin Dm0 in Drosophila embryos by the microinjection of dsRNA. Embryos at 30 minutes old were microinjected with dsRNA specific for the dLBR gene (B-B'', dLBR; A-A'', uninjected control embryo) or lamin Dm0 (D-D'', lamin Dm0; C-C'', uninjected control embryo). Squash preparations of 24-hour-old control and microinjected embryos were stained with antibodies against dLBR (dLBR, A,A',B,B',C',D') or lamin Dm0 (A'',B'',C,D) and the chromatin was stained by Hoechst 33258 (C'',D'', DNA). To demonstrate the degree of the reduction of the nuclear staining of embryos microinjected with dLBR dsRNA, digital images were recorded with a signal enhancement of 647 V (A,B) and 774 V (A',B'). Weak nuclear staining by dLBR antibodies of embryos microinjected with dLBR dsRNA was first visible at a signal enhancement of 774 V (B'). Most of the nuclei shown in D-D'' were ruptured during the squash preparation owing to the depletion of lamin Dm0 (for quantitative data, see Table 3). Digital images were taken by CLSM (A-B'',C,C',D,D') and with a Zeiss Axiophot (C'',D''). Scale bars, 10 µm.
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