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Fig. 1. Targeted disruption of the mouse ephA2 gene. (A) Map of ephA2 targeting vector, ephA2 and recombined loci. PT1.ephA2 consists of a 2.8 kb EcoRI fragment (3' homology) flanking a pMCIneo expression cassette. The black box represents ephA2 exonic sequences that are disrupted by the neo cassette. The gray box between the EcoRI and SacI sites in the genomic locus map represents a unique sequence located outside the targeting vector sequences used to detect homologous recombination events by Southern blot. B, BglII; H, HindIII; R, EcoRI; X, XbaI. (B) Southern blot analysis of tail DNA confirmed the presence of the wild-type allele in wild-type (+/+) and heterozygous (+/–) mice and the presence of the targeted allele in heterozygous (+/–) and null (–/–) mice.





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