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Fig. 5. Binding of At-{alpha}C-Ad and At-AP180 measured by plasmon resonance (BIAcore 3000). The full-length ear construct and the truncated version of At-{alpha}C-Ad were immobilized on CM-5 chips and H6-At-AP180{delta}ENTH was passed over the sensor surface for 1 minute (association) followed by a 1 minute wash with running buffer (dissociation). (A) The full ear construct binds H6-At-AP180{delta}ENTH with high affinity (kD=35 nM) compared with the truncated version At-{alpha}C-h{delta}ear (kD=682 nM). (B) The binding between GST-At-{alpha}C-h-ear and H6-At-AP180{delta}ENTH is concentration dependent as shown by increasing amounts of the At-AP180 construct.





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