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Fig. 6. At-AP180 is a clathrin binding protein. Pull-down assay with clathrin from Arabidopsis cytosol. The clathrin heavy-chain polypeptide (CHC, 180 kDa) is present in the pellet (P) after incubation with GST-At-AP180{delta}ENTH. Mutation of the clathrin binding-motif DLL for alanine (DLL/AAA) did not abolish binding; GST served as the control. Immunoblot with the anti-CHC specific antibody.





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