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Fig. 7. At-AP180 functions as a clathrin assembly-protein. (A) Gefiltration (Superose 6/FPLC) of coat proteins removed from pig brain CCV. The respective antibodies were used to show the separation of endogeneous AP180 in fractions 26-30 from clathrin triskelia in fractions 17-25. (B) Reassembly experiments. Successful assembly of clathrin triskelia occurs only in the presence of plant H₆-At-AP180 ${delta}$ ENTH. Assembly is abolished when DLL is exchanged into alanine (GST-At-AP180 ${delta}$ ENTH-DLL/AAA in a 1:1 stoichiometry). For reassembly, clathrin was incubated either alone or in the presence of half-molar (2:1) and equimolar (1:1) amounts of H₆-At-AP180 ${delta}$ ENTH, and also in its threefold excess (1:3). H₆-At-AP180 ${delta}$ ENTH (DLL) in the same amount as in (1:3) was also incubated alone to show that no unspecific aggregates were present in the pellets (P). Distribution of CHC and the fusion-protein constructs of At-AP180 in (1:1), (1:3), DLL/AAA and DLL are shown on a Coomassie-stained gel and on an immunoblot with the CHC antibody. S, supernatant.

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