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Fig. 4. Morphological and histological changes in pancreatic explants treated with activin A. Activin A, added on day 1 of culture, induces the appearance of a cystic structure within 24 hours (arrow, d2). This structure expands during the following days and peristaltic-like activity of surrounding cells appears on day 5 (see Movie 1, http://jcs.biologists.org/supplemental/). On day 7, Hematoxylin and Eosin staining of sections (bottom panel) shows that the cyst lumen is surrounded by a cylindrical epithelium with villi. Pancreatic tissue is still present, but it is aside of the cyst. Scale bars, 500 µm for upper panels and 100 µm for lower panel.





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