|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 7. Intestinal differentiation in pancreatic explants by activin A depends upon an indirect induction of Shh. (A) Shh expression in pancreatic explants, measured by semi-quantitative RT-PCR as a function of time for 7 days, is induced rapidly after a single addition of activin A (middle panels). This induction of Shh, which does not occur spontaneously with time in culture (upper panels), is inhibited by follistatin (lower panels). (B) Cycloheximide prevents induction of Shh and of the Fabps (measured as described in Fig. 6) by activin A in pancreatic explants. (C) Treatment of pancreatic explants with cyclopamine, a Hedgehog signaling inhibitor, prevents the activin A-induced appearance of the intestinal structure. Treatment of explants with Shh mimics the effect of activin A. Note that the appearance of two cystic structures is not specific of Shh treatment, as it can also be observed after activin A treatment. Pictures were taken on day 4, after a single addition of the agents indicated. Scale bar, 500 µm. (D). Shh induces in pancreatic explants the intestinal markers i-Fabp and l-Fabp. RT-PCR were performed on RNA extracted on day 7 of culture.
|
