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Fig. 2. Time (A) and dose (B) course analyses of the effect of anisomycin on MAP kinase activity. After treatment with anisomycin, cell lysates were prepared and 20 µg protein was separated on 12.5% gel and transferred onto a PVDF membrane. The same membrane was probed and reprobed after stripping with antibodies against doubly phosphorylated p38 MAP kinase, phosphorylated p46/p54 and ß-actin as control in protein loading. These results were representative of three experiments, each performed with a different preparation of cells, and are expressed as the fold activity (means±s.e.m.) of band density relative to that of untreated control by densitometric analysis.
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