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Fig. 1. Nuage clusters are stable. Panels A-D show VasGFP in the same live-egg chamber at 0, 4, 8 and 12 minutes after the start of imaging. Arrowheads indicate nuage clusters that are stable over this time period. Arrows indicate a nuage cluster that appears to detach from the nuclear envelope and move freely in the nurse cell cytoplasm. Note that this detachment was a very rare event. The sampling rate for this experiment was one scan every 2 minutes, and was 1-2 scans/minute for others. To address the possibility that some clusters are rapidly lost, followed by rapid reassembly of novel clusters at the same positions, some experiments were performed with sampling rates of up to 73 scans/minute, and the clusters appeared stable over 61 frames. Scale bars, 10 µm.
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