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Fig. 4. Bruno is a nuage component. Immunostaining shows (A,E) Bru protein, (B,F) GFPAub and (D) BruGFP. (C) Bru staining in red and GFPAub in green. Bru protein can be found partially concentrated in particles around the nucleus (A, and red in C), where it colocalizes with GFPAub (B, and green in C). The colocalization of these molecules is not complete, suggesting some heterogeneity in the nuage. BruGFP is also found in these foci (D). This concentration is largely abolished in vas^AS/vas^AS nurse cells (E). Nuage, visualized with GFPAub, does not appear disrupted in aret^QB/aret^QB pseudo-egg chambers (F), or in the nurse cells of the weaker allelic combination of aret^PA/aret^PD (data not shown). Scale bars, 10 µm (A-D and F), 20 µm (E).

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