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Fig. 6. Nuage-particle movements in the oocyte. (A) To track oocyte-particle movements, GFPAub was imaged in a live stage-8/9 egg chamber for 60 minutes (
t=1 minute) in multiple z-planes, with the data shown as a combined z-axis and time series projection. The arrows are positioned next to a subset of the moving particles and show the direction and path of movements. (B-D) GFPAub in different regions of a single egg chamber, images were taken from a series of scans performed at one-minute intervals. B and C are the first and last scans of the series and show that the movement of nuage particles follow general cytoplasmic movements. The arrows show the direction of and the distance covered by a nuage particle (small arrow) and a yolk platelet/vesicle (large arrow) in this time interval. The yolk platelet/vesicles (which appear black because they lack cytoplasmic GFPAub fluorescence) provide a simple means of tracking general cytoplasmic movements. D shows that particle movements near the posterior pole of the oocyte particle movements are restricted. The arrow shows the rapid movement of a nuage particle at the anterior (the same particle whose movement is shown by the small arrows in B and C), whereas a particle close to the posterior (arrowhead) is relatively immobile. These results are typical of those obtained in multiple additional experiments. The dashed line in A delineates the oocyte nurse-cell boundary. Scale bars, 7 µm (B-D) and 10 µm (A).
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