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Fig. 8. Oskar nucleates the formation of exogenous GFPAub particles in the early oocyte. Osk protein in wild-type egg chambers (A) and in flies expressing osk-bcd3'UTR (B). Arrows in B indicate ectopic Osk protein expressed from the osk-bcd 3'UTR transgene, arrows in A show wild-type oocytes with no Osk staining (the follicle cell staining is non-specific). (C-F) GFPAub fluorescence in wild-type (C) and osk-bcd 3'UTR flies (D-F). Wild-type oocytes contain very few GFPAub particles (arrow in C), but those with the osk-bcd 3' UTR transgene have abundant particulate GFPAub fluorescence (arrows in D,E), as well as a significantly enhanced level of cytoplasmic GFPAub. The image in F is a combined z-axis and time projection of a live stage-7 osk-bcd 3' UTR egg chamber that has been imaged at 70-seconds intervals for 14 frames, showing that very few nuage particles can be tracked entering the early oocyte, just as in wild type. Dashed lines in C, D, and F delineate the oocyte-nurse cell boundaries. Scale bars, 5 µm (E), 15 µm (D,F), 17 µm (B), 20 µm (C) and 23 µm (A).
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