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Fig. 9. Oskar protein promotes ectopic particle formation in nurse cells. Osk protein in wild-type (A) and UASosk egg chambers (B). Ectopic Osk protein is present at the cortex of oocytes and in nurse cells of UASosk egg chambers. (C,D) Distribution of GFPAub in UASosk egg chambers. Clumps of GFPAub particles are often found in the nurse cells of these egg chambers (arrows in C,D), and the level of GFPAub fluorescence is often enhanced. (E-G) The extent of colocalization of GFPAub and Osk was monitored by double labeling of UASosk egg chambers. Osk (G) and GFPAub (F) are merged in E, with Osk in red and GFPAub in green. The clumps of GFPAub particles colocalize with regions of concentrated Osk protein (arrows in E-G). Most particles in these clumps contain GFPAub and Osk (yellow particles in E, short arrows in E-G), some particles contain mostly Osk protein (red particles in E), others only contain GFPAub (long arrows in E-G), particularly those attached to the nuclear envelope (arrowheads in E-G). Osk protein was distributed throughout the nurse cells, but was not found to be strongly associated with the nurse-cell cortex, in contrast to the strong cortical association within the oocyte (B,G). Scale bars are 10 µm (D-G), 20 µm (C) and 100 µm (A,B).
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