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Fig. 4. Effect of myosin VIIa tail overexpression on the entry of InlA- and InlB-coated beads, L. monocytogenes {Delta}inlA and {Delta}inlB. (A) L2071 hEcad overexpressing the GFP/myosin-VIIa-tail were incubated with InlA- or InlB-coated beads. Arrows show sites where the GFP/myosin-VIIa-tail is recruited around entering InlA-coated beads. No recruitment was observed around InlB-coated beads. Boxed regions (left) are enlarged in the middle and on the right. The proportion of InlA- and InlB-coated beads associated with cells that recruit GFP/myosin-VIIa-tail was evaluated and compared with that of InlA-coated beads recruiting GFP alone. (B) L2071 hEcad were transfected with a plasmid expressing GFP alone (L2071 hEcad+GFP) or the tail of myosin VIIa fused to GFP (L2071 hEcad+ GFP-MyoVIIa tail). Entry values are expressed as proportion of intracellular beads or bacteria compared with the total number of beads or bacteria associated with the cells. Results are means±SD, from three independent experiments, each done in triplicate.





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