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Fig. 6. Contribution of the C-terminus of 
-catenin in the recruitment of vezatin at adherens junction and in InlA-mediated entry. (A) Schematic representations of E-cadherin, -catenin and the chimeras fusing the extracellular domain of human E-cadherin (amino acids 1-580) to different regions of the C-terminal domain of -catenin and of the human E-cadherin variant lacking its cytoplasmic tail. Dotted lines indicate the regions of -catenin absent from each construct. (B) Expression level of each chimera in L2071 fibroblasts. Using the HECD1 antibody, the expression level of each chimera (defined by the fluorescence intensity of individual transfected cells) was measured for 150 transfected cells (50 transfected cells per coverslip) and compared with untransfected cells. Values are means±SD of three independent experiments. (C) Localization by fluorescence microscopy of vezatin and human E-cadherin in transfected L2071 cells expressing the different chimeras. (D) Proportion of intracellular beads and bacteria compared with total associated beads or bacteria in L2071 fibroblasts expressing the different chimeras. hEcad cells express the full-length human E-cadherin and hEcad-
cyto cells express a variant of human E-cadherin lacking its cytoplasmic domain. Values are means±SD for three independent experiments, each done in triplicate.
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