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Fig. 1. Effect of type 3 RyR knockdown, RyR inhibition and Ins(1,4,5)P3 R inhibition on early global Ca2+ signaling upon TCR/CD3 ligation. T cells [control clone E2 (a,c,d) or type 3 RyR-knockdown clone 25 (b)] were loaded with Fura-2/AM and analyzed by single-cell Ca2+ imaging as described in the Materials and Methods. OKT3 was added to a final concentration of 10 µg/ml as indicated. Inhibitors were microinjected directly before start of the measurement: (c) Ruthenium Red (RuRed; pipette concentration 10 µM), (d) D-Ins(1,4,6)PS3 (pipette concentration 80 µM). Data acquisition rate was 0.1 ratios/second before OKT3 addition, and 2 ratio/second thereafter. Characteristic tracings out of 18-39 cells are displayed.





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