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Fig. 2. Localization of microinjected probe nanospheres within the cell. Superposition of a phase-contrast micrograph of a cell and a fluorescent micrograph of DAPI-stained DNA (blue) and nanospheres (red) embedded in: (A) the intranuclear region (nanospheres 1-3) and (B) cytoplasm (nanospheres a-c). B was taken at a lower plane of focus than A. (C) Subcellular localization of nuclear (blue) and cytoplasmic (gray) nanospheres shown in A and B. Images represent composite images obtained by superimposing phase-contrast micrographs of the cell with fluorescent micrographs of the nanospheres and nuclear DAPI-stained DNA taken at different planes of focus. (D) Overall shape of the plasma membrane (phase contrast) and nucleus (blue DAPI stain) of the cells used in A-C.





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