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Fig. 4. Ultrastructural identification of internalized FM1-43 stained dense cores. Photoconversion revealed that internalized FM1-43 was located in dense cores that were clustered within a membrane. Dark FM1-43-DAB reaction product (arrows) is clearly distinguished from unlabeled dense core vesicles (arrowheads). FM1-43 was found in vesicles containing cores that appeared to coalesce (A) and in vesicles with multiple distinct cores (B). Bar, 200 nm for both images.
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