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Fig. 1. Effect of preincubation with LPS on the reduction of NBT by DCs challenged with PMA. General views of DCs (A-D) or sDCs (E). Left, phase contrast images; right, NBT staining. (A,E) Preincubation with LPS, followed by incubation with PMA. (B) No LPS in the preincubation medium, incubation with PMA. (C) Preincubation with LPS, no PMA in the incubation medium. (D) Control cells, no LPS in the preincubation medium, no PMA in the incubation medium. Micrographs F, G and H correspond to high magnification images of selected cells from plates A, B and E, respectively. (I) Time course of NBT reduction within DCs. Preincubation with LPS, followed by incubation with PMA ( ${blacktriangleup}$ ). Other conditions: no preincubation with LPS, PMA in the incubation medium ( ${blacksquare}$ ); Preincubation with LPS, no PMA in the incubation medium ( ${diamondsuit}$ ); Control, no preincubation with LPS, no PMA in the incubation medium ( ${circ}$ ). The results are representative of five separate experiments.

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