spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 6. G3{Delta}EGF expression increased and maintained FAK phosphorylation. Cells were cultured in DMEM containing 10% FBS for 24 hours followed by serum withdrawal and incubation in serum-free DMEM for different times. The cell lysate was prepared and immunoprecipitated with anti-FAK antibody, followed by western blotting probed with anti-phosphotyrosine antibody. U87 cells expressing G3{Delta}EGF retained a prolonged FAK phosphorylation (p-FAK) compared with the vector-transfected cells. The same blot was also probed with anti-FAK antibody to assess equal protein loading. Additionally, the cell lysate was analysed on a western blot probed with anti-ß1-integrin antibody. These experiments were repeated three times.





Right arrow Return to article