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Fig. 3. The NRSE-like motif of the mouse complexin I gene binds REST. (a) EMSA with ³²P-labeled CPXI-NRSE using nuclear extracts from HeLa cells. A slow migrating complex (arrow) is observed. This complex was disrupted by REST antibodies but not by unrelated antibodies against transcription factors OCT1, SP1 and CEBPß. (b) This DNA-binding activity was competed by adding a 100- or 600-fold molar excess of unlabeled wild-type NRSE of the MAPK8IP1 gene (WT) previously described to specifically bind REST (Abderrahmani et al., 2001). This binding activity was not disrupted by adding unlabeled mutated NRSE of the MAPK8IP1 gene (MUT). NE, nuclear extracts.

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