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Fig. 2. LMB inhibits the nuclear export of IFN-{alpha}1 mRNA. HeLa cell nuclei were microinjected with either phuIFN-{alpha}1, pCRRE or pRSVluc-CTE at 37°C for 30 minutes as described in the Materials and Methods. The cells were further incubated at 37°C for 30 minutes (A,G; t: 0 for cells expressing IFN-{alpha}1 mRNA and luc-CTE mRNA, respectively) or 45 minutes (D; t: 0 for cells expressing HIV-1 gag mRNA), and then treated for an additional 3 hours with 20 nM LMB (C,F,I; LMB: 3 h) as described in the Materials and Methods. (B,E,H; D10: 3 h) Mock-treated cells. The cells were subsequently fixed, permeabilised and subjected to RNA-FISH as described in the Materials and Methods. Representative examples of each type of injected cell are shown. Scale bar: 10 µm.





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