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Fig. 8. b-24-DsRed cells contain mtATPase oligomers and show abnormal mitochondrial ultrastructure. b-24-DsRed mitochondria were solubilised with dodecyl ß-maltoside (4 g/g protein) and subjected to CN-PAGE. The gel was imaged for DsRed fluorescence (A). Lower-case letters indicate the positions of mtATPase tetramer (a), trimer (b), dimer (c) and monomer (d). TEM was performed on b-24-DsRed sections (B) as described in Materials and Methods. White arrows, normal mitochondria; black arrows, abnormal mitochondria. Images are presented in reverse contrast, with membranes being black. Bars, 0.5 µm.





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