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Fig. 2. The PTAP motif is also required for HTLV-1-Gag release. (A) Schematic representation of the HTLV-1 Gag protein. The sequence of the L domain at the C-terminus of the MA domain is indicated, and the PPPY and PTAP motifs are shown in bold characters. Positions of the introduced Y121D, P124L and P127L mutations are indicated. (B) Immunoprecipitation of HTLV-1 proteins from 293T cells transfected with either the wild-type (WT), PTAP-mutated (P127L) or PPPY-mutated (Y121D) provirus. Virus proteins were immunoprecipitated from either cell lysates (Cells), or from sucrose-cushion purified virions (Virions). Immature (Gagp55) and mature (CAp24, MAp19 and NCp15) Gag products are indicated. (C) Anti-MAp19 ELISA was used to quantify virus particles released from 293T cells transfected with either the wild-type (WT), PTAP-mutated (P127L) or PPPY-mutated (Y121D) provirus. The results are the means and standard errors of three independent experiments.





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