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Fig. 3. Tsg101 is required for HTLV-1 release. (A) Anti-Tsg101 immunoblot analysis of 293T cells transfected with either control (ctrl) or Tsg101-specific siRNAs. (B) Anti-MAp19 ELISA was used to quantify wild-type virus release from 293T cells after depletion of Tsg101 by siRNAs. For comparison, the amount of virus particles produced by the PTAP-mutated provirus (P127L) is indicated. For each condition, Gag intracellular production and extracellular virion-associated CAp24 as detected by immunoblots are also shown. The ELISA results are the means and standard errors of at least three independent experiments, and immunoblots correspond to one representative experiment.





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