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Fig. 4. Wild-type HTLV-1-Gag proteins localize mainly in the endocytic pathway in 293T cells at steady state. (A) Gag co-staining with the whole endocytic pathway revealed by 1-hour uptake of FITC-conjugated dextran. (B) Gag co-staining with early/recycling endosomes revealed by 1-hour uptake of cyanin 3-conjugated transferrin. (C) Gag co-staining with the late endosomes/MVBs marker CD63. Each row shows a median section of cells recorded by confocal microscopy using a 63x objective and a 4x zoom. Overlays of red and green pictures are shown as well as bright light pictures. Zoomed images of colocalization areas are also presented (boxed).